GLP-1R positive nerve fibres (Abcam antibody, left panels) and PGP9.5 (right panels) in mucosal sigmoid colon biopsy specimens, from top-control, middle and bottom-IBD, magnification x40. GLP-1R expressing nerve fibres were increased in IBD compared to controls. 0.0013), that were similar in the UC and daily dietary supplement CD pools (Fig 2B) compared to controls. 0.6), suggesting an overall increase in expression of neuronal fibres expressing GLP-1R in the IBD group. Bar charts showing an overall increase in expression of nerve fibres expressing GLP-1R in the IBD group by image analysis of GLP-1R (A, B) and PGP9.5 (C) in colonic biopsies (mean ± SEM, Mann Whitney U test). There was no difference in the ratio of GLP-1R to PGP9.5, indicating an overall increase in the neuronal fibres expressing GLP-1R. CGRP neuronal staining (arrowed) in mucosal sigmoid colon biopsy specimens from control (A), IBD (B and C), magnification x40. Bar chart showing image analysis of CGRP in colonic biopsies (mean ± SEM, Mann Whitney U test) (D).

GLP-1R (Abcam antibody, E), and PGP9.5 (F), staining in avulsion injury DRG, magnification x20. Serial sections of GLP-1R-immunoreactive DRG immunostained with no peptide (A) or with 1 μg peptide (B), 100 ng peptide (C), 10 ng peptide (D), 1 ng peptide (E) or 100 pg peptide (F); Abcam antibody at a dilution of 1:1500; magnification x40. Serial sections of GLP-1R immunoreactivity in sub-mucous plexus of a full thickness normal colon immunostained with no peptide (A) or with 1 μg peptide (B), 100 ng peptide (C), 10 ng peptide (D), 1 ng peptide (E) or 100 pg peptide (F) Abcam antibody at a dilution of 1:1500; magnification x40. No specific staining was seen with either GLP-1R antibody in the HEK negative cell lines, MedicGLP or when primary antibody was omitted. In contrast, in the GLP-1R transfected cell lines both antibodies gave strong staining in these cells, with appropriate serial dilution effects (S1 and S2 Figs). Double staining of GLP-1R (Abcam GLP-1R antibody at a dilution of 1:1000, black) with the neuronal marker β Tubulin (at a dilution of 1:1000, red) in, A, sub-mucous plexus of colon from a patient with IBD and, B, human DRG; magnification x20. 0.016). Staining with this antibody to GLP-1R (Acris) was best observed at dilution of 1:100; the immunoreactivity diminished with increasing antibody dilution. This antibody detects endogenous levels of total GLP-1R protein, and regarding specificity the synthetic peptide abolished immunofluorescence staining of HeLa cells (Acris source data sheet).

Science continues to prove that the old adage "you are what you eat" is profoundly true. This is because you have trillions of microorganisms living in your gut, which are directly responsible for MedicGLP Formula nearly every aspect of your health. These microbes make up what’s called your "gut microbiota." They are especially influential in determining your likelihood of gaining excessive weight, becoming obese, and developing obesity-related diseases like diabetes, heart disease, and premature death. The gut microbiome (the expressed genes of your gut microbiota) plays a major role in your metabolism through energy production, storage, and expenditure. So, it’s no surprise that science has found a strong connection between gut microbiome imbalance and metabolic syndrome. Yikes! If you have the wrong mix of microbes, it could be making it tough for you to get your health on track! Did you know: Scientists can actually look at your gut microbiome composition and tell with 90% accuracy if you are obese or lean. That’s pretty impressive, isn’t it?

1. Unable to lose weight and keep it off? 2. Struggling to fight cravings? 3. Sick of starving yourself for weight loss? 4. Baffled as to why no diet seems to really work for you? Depending on the composition of your gut microbiome, your microbes can either boost your health or weigh you down (figuratively and literally). The good news is this: You have control over these little guys with every bite you eat. Diet has the power to change the landscape of your gut microbiome. So, if you’ve tried multiple diets and can’t seem to get the results you want, it’s time to look into your gut microbiome.Will You Lose Weight Naturally on an Individualized Diet? If you’ve been struggling to lose weight and keep it off - it could be your gut microbiome. While Viome doesn’t guarantee weight loss, it has been an exciting result many of our customers have reported. "Fiona’s update day 37!